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Axonal Guidance Proteins
Anti-Dscam Antibody (A83347)
This product is identical to Novus (NB100-41390). Discover more for less.
Overview
Specifications
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$480
Product Datasheet
Goat polyclonal antibody to Dscam for ELISA, WB, IF and Flow Cytometry.
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Product Size:
100µg
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Shipping Information
Delivery
Freight Charges
$40 to United States
Lead Time
3-5 Business Days
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Telephone
+1 (314) 370-6046
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Specifications
Name
Anti-Dscam Antibody
Description
Goat polyclonal antibody to Dscam.
Applications
ELISA
,
WB
,
IF
,
Flow Cytometry
Dilutions
ELISA: 1:16,000, WB: 2-3µg/ml, IF: 10µg/ml, Flow Cytometry: 10µg/ml
Reactivity
Human
Immunogen
Synthetic peptide corresponding to the internal region of human Dscam.
Sequence
C-DSWDSAQRTKDVSPQ
Host
Goat
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 µg/ml
Molecular Weight
200-220 kDa
Predicted MW
174kDa
Product Form
Liquid
Formulation
Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
CHD2, CHD2 42, CHD2 52, Down syndrome cell adhesion molecule, DSCAM_HUMAN, human CHD2-52 down syndrome cell adhesion molecule 2, 9, OTTHUMP00000109193 2, SD11109p
Isotype Controls
Goat IgG (A121671)
Suitable Secondaries
Donkey Anti-Goat IgG H&L Antibody (AP) (A300679)
Donkey Anti-Goat IgG H&L Antibody (Biotin) (A300716)
Donkey Anti-Goat IgG H&L Antibody (FITC) (A300685)
Donkey Anti-Goat IgG H&L Antibody (HRP) (A300730)
See all Anti-Goat IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Scientific Validation Data (3)
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Figure 1: Western Blot - Anti-Dscam Antibody (A83347)
Anti-DSCAM Antibody (A83347) (2µg/ml) staining of HepG2 cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
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Figure 2: Immunofluorescence - Anti-Dscam Antibody (A83347)
Anti-DSCAM Antibody (A83347) - Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10µg/ml) followed by Alexa Fluor 488 secondary antibody (2µg/ml), showing nuclear staining.
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Figure 3: Flow Cytometry - Anti-Dscam Antibody (A83347)
Anti-DSCAM Antibody (A83347) - Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10µg/ml) followed by Alexa Fluor 488 secondary antibody (1µg/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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