Anti-Doublecortin Antibody (A83041)

Doublecortin expression in Mouse fetal Brain lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-Doublecortin Antibody (A83041) at 0.5µg/ml and detected by chemiluminescence.

Doublecortin expression in HepG2 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-Doublecortin Antibody (A83041) at 5µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Cytoplasmic staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 1µg/ml.