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Anti-Doublecortin Antibody (A13291)

Western Blot - Anti-Doublecortin Antibody (A13291) - Antibodies.com Immunofluorescence - Anti-Doublecortin Antibody (A13291) - Antibodies.com
$335
Product Datasheet

Rabbit polyclonal antibody to Doublecortin for WB and ICC/IF.

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$40 to United States
Lead Time
5-8 Business Days

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Name
Anti-Doublecortin Antibody
Description
Rabbit polyclonal antibody to Doublecortin.
Applications
WB, ICC/IF
Dilutions
WB: 1:500-1:1,000, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 265-365 of human Doublecortin (NP_835365.1).
Sequence
SLDENECRVMKGNPSATAGPKASPTPQKTSAKSPGPMRRSKSPADSGNDQDANGTSSSQLSTPKSKQSPISTPTSPGSLRKHKDLYLPLSLDDSDSLGDSM
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
45 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
DBCN, Dbct, DC, DCX, DCX_HUMAN, Doublecortex, Doublin, FLJ51296, Lis X, Lis-X, Lissencephalin X, Lissencephalin-X, Lissencephaly X linked, Lissencephaly X linked doublecortin, LISX, Neuronal migration protein doublecortin, OTTHUMP00000023859, OTTHUMP00000023860, OTTHUMP00000216315, OTTHUMP00000216316, SCLH, XLIS
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-Doublecortin Antibody (A13291) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-Doublecortin Antibody (A13291)
Western blot analysis of extracts of various cell lines, using Anti-Doublecortin Antibody (A13291) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.
Immunofluorescence - Anti-Doublecortin Antibody (A13291) - Antibodies.comEnlarge Image
Figure 2: Immunofluorescence - Anti-Doublecortin Antibody (A13291)
Immunofluorescence analysis of Neuro-2a cells using Anti-Doublecortin Antibody (A13291) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).
Publishing research using Anti-Doublecortin Antibody (A13291)? Please let us know so that we can list the citation on this page.

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