Anti-Doublecortin Antibody [3E1] (A85376)

Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat stained with Anti-Doublecortin Antibody [3E1] (A85376), dilution 1:1,000, in red, and co-stained with Anti-MAP2 Antibody (A85363), dilution 1:10,000, in green. The blue is DAPI staining of nuclear DNA. The doublecortin antibody reveals strong cytoplasmic staining in a population of small developing neurons and their processes, while the MAP2 antibody stains dendrites and perikarya of mature neurons. Doublecortin antibody is an excellent marker of early developing neuronal cells.

Western blot analysis of rat whole brain lysates using Anti-Doublecortin Antibody [3E1] (A85376), dilution 1:1,000, in green. The lanes contain: [1] protein standard (red), [2] adult rat brain, [3] embryonic E20 rat brain. The strong bands at 40 kDa and 45 kDa correspond to the doublecortin protein, detected only in the developing brain.