Anti-Dopamine Transporter Antibody (A91403)

Name: Anti-Dopamine Transporter Antibody
Brand: Antibodies.com
SKU: A91403
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to Dopamine Transporter for WB, IHC and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Specifications

Name

Anti-Dopamine Transporter Antibody

Description

Rabbit polyclonal antibody to Dopamine Transporter.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:100, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 500 to the C-terminus of human Dopamine Transporter/SLC6A3 (NP_001035.1).

Sequence

GVGQFSDDIQQMTGQRPSLYWRLCWKLVSPCFLLFVVVVSIVTFRPPHYGAYIFPDWANALGWVIATSSMAMVPIYAAYKFCSLPGSFREKLAYAIAPEKDRELVDRGEVRQFTLRHWLKV

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

72 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

DA transporter, DAT, DAT1, SLC6A3, Sodium-dependent dopamine transporter, Solute carrier family 6 member 3

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - Anti-Dopamine Transporter Antibody (A91403)

Western blot analysis of Rat brain, using Anti-Dopamine Transporter Antibody (A91403) at 1:700 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Immunohistochemistry - Anti-Dopamine Transporter Antibody (A91403)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-Dopamine Transporter Antibody (A91403) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Immunofluorescence - Anti-Dopamine Transporter Antibody (A91403)

Immunofluorescence analysis of rat brain using Anti-Dopamine Transporter Antibody (A91403) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Immunofluorescence - Anti-Dopamine Transporter Antibody (A91403)

Immunofluorescence analysis of mouse brain using Anti-Dopamine Transporter Antibody (A91403) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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