Donkey F(ab')2 Anti-Rabbit IgG H&L Antibody (AP), Cross-Adsorbed (A294929)

Donkey F(ab')2 Anti-Rabbit IgG H&L Antibody (AP), Cross-Adsorbed

Donkey F(ab')2 anti-rabbit IgG H&L (AP) secondary antibody.

Donkey

Rabbit

WB, IHC, ICC, ELISA

ELISA: 1:1,000-1:20,000; ELISA (capture antibody): 1:50-1:250, ICC: 1:200-1:2,000, IHC: 1:200-1:2,000, WB: 1:1,000-1:20,000

Polyclonal

IgG

AP

0.5 mg/ml

Antigen affinity purification.

Antigen affinity purification.

Supplied in 50 mM HEPES, pH 7.1, with 0.1 M Sodium Chloride, 1 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 0.2% BSA, and 0.09% Sodium Azide.

Store in the dark at 4°C. Avoid prolonged exposure to light. Do not freeze!

Antiserum was cross-adsorbed using bovine, chicken, goat, human, mouse, rat, and sheep immunosorbents to remove cross reactive antibodies. This antibody was then isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion, and Fc fragments and whole IgG molecules were removed. F(ab')2 fragments were then conjugated to alkaline phosphatase (AP).

This product is for research use only. It is not intended for diagnostic or therapeutic use.