Anti-Dnmt1 Antibody (A91686)

Name: Anti-Dnmt1 Antibody
Brand: Antibodies.com
SKU: A91686
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to Dnmt1 for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-Dnmt1 Antibody

Description

Rabbit polyclonal antibody to Dnmt1.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-124 of DNMT1 (NP_001370.1).

Sequence

MPARTAPARVPTLAVPAISLPDDVRRRLKDLERDSLTEKECVKEKLNLLHEFLQTEIKNQLCDLETKLRKEELSEEGYLAKVKSLLNKDLSLENGAHAYNREVNGRLENGNQARSEARRVGMAD

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

230 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

ADCADN, AIM, CXXC finger protein 9, CXXC-type zinc finger protein 9, CXXC9, DNA (cytosine 5 ) methyltransferase 1, DNA (cytosine-5)-methyltransferase 1, DNA methyltransferase 1, DNA methyltransferase HsaI, DNA methyltransferase M.HsaI., DNA MTase, DNA MTase HsaI, DNMT, DNMT 1, Dnmt1o, DNMT1_HUMAN, FLJ16293, HSN1E, M.HsaI, MCMT, Met1, MGC104992, mMmul, MommeD2

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-Dnmt1 Antibody (A91686)

Western blot analysis of extracts of various cell lines, using Anti-Dnmt1 Antibody (A91686) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Figure 2: Immunohistochemistry - Anti-Dnmt1 Antibody (A91686)

Immunohistochemistry analysis of paraffin-embedded human spleen using Anti-Dnmt1 Antibody (A91686) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 3: Immunofluorescence - Anti-Dnmt1 Antibody (A91686)

Confocal immunofluorescence analysis of HeLa cells using Anti-Dnmt1 Antibody (A91686) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).

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Figure 4: Immunofluorescence - Anti-Dnmt1 Antibody (A91686)

Confocal immunofluorescence analysis of HeLa cells using Anti-Dnmt1 Antibody (A91686) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).

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