Anti-Dnmt1 Antibody (A307715)

Name: Anti-Dnmt1 Antibody
Brand: Antibodies.com
SKU: A307715
Price: 335.0 USD
Availability: InStock

Enlarge Image

$335

Rabbit polyclonal antibody to Dnmt1 for WB, ICC/IF, IP and ChIP.

100% Guarantee

Price Match Guarantee

Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

Contact Us

Telephone

+1 (314) 370-6046

orders@antibodies.com

Guarantees

100% Quality Guarantee

Price Match Guarantee

Specifications

Name

Anti-Dnmt1 Antibody

Description

Rabbit polyclonal antibody to Dnmt1.

Applications

WB, ICC/IF, IP, ChIP

Dilutions

WB: 1:500-1:2,000, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000, ChIP: 1:500-1:1,000

Reactivity

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1417-1616 of human DNMT1 (NP_001370.1).

Sequence

MSALVAARMRHIPLAPGSDWRDLPNIEVRLSDGTMARKLRYTHHDRKNGRSSSGALRGVCSCVEAGKACDPAARQFNTLIPWCLPHTGNRHNHWAGLYGRLEWDGFFSTTVTNPEPMGKQGRVLHPEQHRVVSVRECARSQGFPDTYRLFGNILDKHRQVGNAVPPPLAKAIGLEIKLCMLAKARESASAKIKEEEAAKD

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

ADCADN, AIM, CXXC finger protein 9, CXXC-type zinc finger protein 9, CXXC9, DNA (cytosine 5 ) methyltransferase 1, DNA (cytosine-5)-methyltransferase 1, DNA methyltransferase 1, DNA methyltransferase HsaI, DNA methyltransferase M.HsaI., DNA MTase, DNA MTase HsaI, DNMT, DNMT 1, Dnmt1o, DNMT1_HUMAN, FLJ16293, HSN1E, M.HsaI, MCMT, Met1, MGC104992, mMmul, MommeD2

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

Enlarge Image

Figure 1: Western Blot - Anti-Dnmt1 Antibody (A307715)

Western blot analysis of extracts of various cell lines, using Anti-Dnmt1 Antibody (A307715) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

Enlarge Image

Figure 2: Immunofluorescence - Anti-Dnmt1 Antibody (A307715)

Immunofluorescence analysis of 293T cells using Anti-Dnmt1 Antibody (A307715) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

Enlarge Image

Figure 3: Western Blot - Anti-Dnmt1 Antibody (A307715)

Immunoprecipitation analysis of 300µg extracts of Jurkat cells using 3µg of Anti-Dnmt1 Antibody (A307715). This Western blot was performed on the immunoprecipitate using Anti-Dnmt1 Antibody (A307715) at a dilution of 1:1000.

Enlarge Image

Figure 4: Chromatin Immunoprecipitation - Anti-Dnmt1 Antibody (A307715)

Chromatin immunoprecipitation (ChIP) analysis of extracts of Jurkat cells, using Anti-Dnmt1 Antibody (A307715) and Rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Publishing research using Anti-Dnmt1 Antibody (A307715)? Please let us know so that we can list the citation on this page.