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Anti-DNA polymerase I Antibody (A264)

Western blot analysis of DNA polymerase 1 in crude extract of E. coli cells by using Anti-DNA polymerase 1 Antibody. 1: Purified E. coli DNA polymerase 1 (10ng). 2: Sonic lysate supernatant of E. coli strain AB1157 cells. Primary antibody was used at 1:2,000 dilution. Predicted molecular mass of DNA polymerase 1 is 103 kDa. Immunoprecipitation of DNA polymerase 1 from crude lysate of E. coli with Anti-DNA polymerase 1 Antibody. 1: Purified DNA polymerase 1 (20ng), WB. 2: Purified DNA polymerase 1 (10ng), WB. 3: Crude lysate of E. coli strain AB1157 (10µg), WB. 4: The crude lysate of E. coli cells was reacted with Anti-DNA polymerase 1 Antibody and precipitated with protein G conjugated magnetic beads, and analyzed by WB by using Anti-DNA polymerase 1 Antibody. As the secondary antibody, Anti-Rabbit IgG Antibody (HRP) for IP was used.
$535

Rabbit polyclonal antibody to DNA polymerase I for WB and IP.

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Freight Charges
$40 to United States
Lead Time
4-6 Business Days

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+1 (314) 370-6046
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Name
Anti-DNA polymerase I Antibody
Description
Rabbit polyclonal antibody to DNA polymerase I.
Applications
WB, IP
Dilutions
WB: 1:1,000-1:5,000
Reactivity
Escherichia coli
Immunogen
Recombinant full length E. coli DNA polymerase I.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Product Form
Liquid
Formulation
Supplied as antiserum with 0.05% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
POL I, pol1, polA, resA, Taq polymerase 1
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western blot analysis of DNA polymerase 1 in crude extract of E. coli cells by using Anti-DNA polymerase 1 Antibody. 1: Purified E. coli DNA polymerase 1 (10ng). 2: Sonic lysate supernatant of E. coli strain AB1157 cells. Primary antibody was used at 1:2,000 dilution. Predicted molecular mass of DNA polymerase 1 is 103 kDa.Enlarge Image
Figure 1: Anti-DNA polymerase I Antibody (A264)
Anti-DNA polymerase I Antibody
Immunoprecipitation of DNA polymerase 1 from crude lysate of E. coli with Anti-DNA polymerase 1 Antibody. 1: Purified DNA polymerase 1 (20ng), WB. 2: Purified DNA polymerase 1 (10ng), WB. 3: Crude lysate of E. coli strain AB1157 (10µg), WB. 4: The crude lysate of E. coli cells was reacted with Anti-DNA polymerase 1 Antibody and precipitated with protein G conjugated magnetic beads, and analyzed by WB by using Anti-DNA polymerase 1 Antibody. As the secondary antibody, Anti-Rabbit IgG Antibody (HRP) for IP was used.Enlarge Image
Figure 2: Immunoprecipitation - Anti-DNA polymerase I Antibody (A264)
Anti-DNA polymerase I Antibody
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