Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
FLJ16395, FLJ21978, Polh, POLH_HUMAN, polymerase DNA directed eta, RAD30, RAD30 homolog A, RAD30A, Xeroderma pigmentosum variant type protein, XP V, XPV
Figure 1: Western Blot - Anti-DNA polymerase eta Antibody (A13688)
Western blot analysis of extracts of various cell lines, using Anti-DNA polymerase eta Antibody (A13688) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
Figure 2: Immunofluorescence - Anti-DNA polymerase eta Antibody (A13688)
Immunofluorescence analysis of U-2 OS cells using Anti-DNA polymerase eta Antibody (A13688) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
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