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Anti-DNA polymerase eta Antibody [5H10] (A358)

Identification of Pol eta in whole cell extract of HeLa cells by western blot with Anti-DNA polymerase eta Antibody. Lane 1: Recombinant full-size Pol eta with His6 tag at C-teminus (Positive Control). Lane 2: Whole cell extract of HeLa cells (40µg) (Positive Control). Lane 3: Whole cell extract of XP2SA (XP-V) cells (40µg) (Negative Control). Pol eta is detected at ~80 kDa position. 8% gel was used. Immunofluorescence staining of DNA polymerase eta in HeLa cells with Anti-DNA polymerase eta Antibody. HeLa cells were fixed in 4% paraformaldehyde overnight and permeabilized in 0.25% TritonX 100 in PBS for 10 min. Anti-DNA polymerase eta Antibody was used at 1:1,000 dilution.
$535

Mouse monoclonal (5H10) antibody to DNA polymerase eta for WB and IF.

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Freight Charges
$40 to United States
Lead Time
4-6 Business Days

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Name
Anti-DNA polymerase eta Antibody [5H10]
Description
Mouse monoclonal (5H10) antibody to DNA polymerase eta.
Applications
WB, IF
Dilutions
WB: 1:500-1:2,000, IF: 1:200-1:1,000
Reactivity
Human
Immunogen
Recombinant full length human Pol eta protein (His tag).
Host
Mouse
Clonality
Monoclonal
Clone ID
5H10
Isotype
IgG1
Conjugate

Unconjugated

Purification
Ion exchange chromatography.
Concentration
1 mg/ml
Purity
> 90% (by SDS-PAGE)
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 50% Glycerol (filter sterilised, without Sodium Azide, and carrier free).
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
FLJ16395, FLJ21978, Polh, POLH_HUMAN, polymerase DNA directed eta, RAD30, RAD30 homolog A, RAD30A, Xeroderma pigmentosum variant type protein, XP V, XPV
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Identification of Pol eta in whole cell extract of HeLa cells by western blot with Anti-DNA polymerase eta Antibody. Lane 1: Recombinant full-size Pol eta with His6 tag at C-teminus (Positive Control). Lane 2: Whole cell extract of HeLa cells (40µg) (Positive Control). Lane 3: Whole cell extract of XP2SA (XP-V) cells (40µg) (Negative Control). Pol eta is detected at ~80 kDa position. 8% gel was used.Enlarge Image
Figure 1: Anti-DNA polymerase eta Antibody [5H10] (A358)
Anti-DNA polymerase eta Antibody
Immunofluorescence staining of DNA polymerase eta in HeLa cells with Anti-DNA polymerase eta Antibody. HeLa cells were fixed in 4% paraformaldehyde overnight and permeabilized in 0.25% TritonX 100 in PBS for 10 min. Anti-DNA polymerase eta Antibody was used at 1:1,000 dilution.Enlarge Image
Figure 2: Immunofluorescence - Anti-DNA polymerase eta Antibody [5H10] (A358)
Anti-DNA polymerase eta Antibody
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