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Anti-DNA Polymerase epsilon p59 Antibody (A90627)

Western Blot - Anti-DNA Polymerase epsilon p59 Antibody (A90627) - Antibodies.com
$335
Product Datasheet

Rabbit polyclonal antibody to DNA Polymerase epsilon p59 for WB.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

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+1 (314) 370-6046
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Name
Anti-DNA Polymerase epsilon p59 Antibody
Description
Rabbit polyclonal antibody to DNA Polymerase epsilon p59.
Applications
WB
Dilutions
WB: 1:500-1:2,000
Reactivity
Human, Mouse
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-290 of human POLE2 (NP_002683.2).
Sequence
MAPERLRSRALSAFKLRGLLLRGEAIKYLTEALQSISELELEDKLEKIINAVEKQPLSSNMIERSVVEAAVQECSQSVDETIEHVFNIIGAFDIPRFVYNSERKKFLPLLMTNHPAPNLFGTPRDKAEMFRERYTILHQRTHRHELFTPPVIGSHPDESGSKFQLKTIETLLGSTTKIGDAIVLGMITQLKEGKFFLEDPTGTVQLDLSKAQFHSGLYTEACFVLAEGWFEDQVFHVNAFGFPPTEPSSTTRAYYGNINFFGGPSNTSVKTSAKLKQLEEENKDAMFVFL
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
59 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
DNA polymerase epsilon subunit 2, DNA polymerase epsilon subunit B, DNA polymerase II subunit 2, DPE 2, DPE2, DPOE2_HUMAN, POLE 2, POLE2, Polymerase (DNA directed) epsilon 2, Polymerase (DNA directed) epsilon 2 (p59 subunit), polymerase (DNA directed), epsilon 2, accessory subunit, Polymerase, DNA, epsilon-2
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-DNA Polymerase epsilon p59 Antibody (A90627) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-DNA Polymerase epsilon p59 Antibody (A90627)
Western blot analysis of extracts of various cell lines, using Anti-DNA Polymerase epsilon p59 Antibody (A90627) at 1:3,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
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