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Anti-DNA Photolyase Antibody (A101676)

Western blot of E. coli photolyase. 1. Purified Photolyase (8.4ng). 2. Purified Photolyase (42ng). 3. E. coli crude lysate (40µg). SDS-PAGE 12.5% gel. Blotting: 15 v, overnight, blocking by 5% skim milk. Anti-DNA Photolyase Antibody was used at 1:1,000 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H&L) was used at 1:10,000. Note that there are only 10-20 molecules of photolyase per E. coli cell. Immunoprecipitation of E. coli Photolyase. Immunoprecipitation was performed by using Dynabeads Magnetic system (Thermo Fisher). 1: E. coli AB1157 cell lysate (300µg), Anti-DNA Photolyase Antibody (2µg). 2: E. coli AB1157 cell lysate (300µg), Anti-DNA Photolyase Antibody (10µg). 3: Positve control: E. coli Photolyase purified (4.2µg), Anti-DNA Photolyase Antibody (10µg). 4: Negative control: No sample, Anti-DNA Photolyase Antibody (10µg). Western blot: Anti-DNA Photolyase Antibody was used at 1:1,000 dilution and the secondary antibody was Goat Anti-Rabbit IgG (HRP) used at 1:1,000 dilution.
$535

Rabbit polyclonal antibody to DNA Photolyase for WB, IP and ELISA.

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Freight Charges
$40 to United States
Lead Time
4-6 Business Days

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Name
Anti-DNA Photolyase Antibody
Description
Rabbit polyclonal antibody to DNA Photolyase.
Applications
WB, IP, ELISA
Dilutions
WB: 1:1,000, IP: 1:200
Reactivity
Escherichia coli
Immunogen
Recombinant full length E. coli Cyclobutane Pyrimidine Dimer Photolyase.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Protein A chromatography.
Concentration
2 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 50% Glycerol (filter sterilised and without Sodium Azide).
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western blot of E. coli photolyase. 1. Purified Photolyase (8.4ng). 2. Purified Photolyase (42ng). 3. E. coli crude lysate (40µg). SDS-PAGE 12.5% gel. Blotting: 15 v, overnight, blocking by 5% skim milk. Anti-DNA Photolyase Antibody was used at 1:1,000 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H&L) was used at 1:10,000. Note that there are only 10-20 molecules of photolyase per E. coli cell.Enlarge Image
Figure 1: SDS-PAGE - Anti-DNA Photolyase Antibody (A101676)
Anti-DNA Photolyase Antibody
Immunoprecipitation of E. coli Photolyase. Immunoprecipitation was performed by using Dynabeads Magnetic system (Thermo Fisher). 1: E. coli AB1157 cell lysate (300µg), Anti-DNA Photolyase Antibody (2µg). 2: E. coli AB1157 cell lysate (300µg), Anti-DNA Photolyase Antibody (10µg). 3: Positve control: E. coli Photolyase purified (4.2µg), Anti-DNA Photolyase Antibody (10µg). 4: Negative control: No sample, Anti-DNA Photolyase Antibody (10µg). Western blot: Anti-DNA Photolyase Antibody was used at 1:1,000 dilution and the secondary antibody was Goat Anti-Rabbit IgG (HRP) used at 1:1,000 dilution.Enlarge Image
Figure 2: Immunoprecipitation - Anti-DNA Photolyase Antibody (A101676)
Anti-DNA Photolyase Antibody
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