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Anti-DLC1 (Isoforms 1 and 3) Antibody (A83189)
This product is identical to Abcam (ab21200). Discover more for less.
Overview
Specifications
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$480
Product Datasheet
Goat polyclonal antibody to DLC1 (Isoforms 1 and 3) for ELISA, IF and IHC.
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Product Size:
100µg
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Freight Charges
$40 to United States
Lead Time
3-5 Business Days
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Specifications
Name
Anti-DLC1 (Isoforms 1 and 3) Antibody
Description
Goat polyclonal antibody to DLC1 (Isoforms 1 and 3).
Applications
ELISA
,
IF
,
IHC
Dilutions
ELISA: 1:64,000, IHC: 4µg/ml, IF: 10µg/ml
Reactivity
Human, Mouse
Immunogen
Synthetic peptide corresponding to the N terminal region of human DLC1 (Isoforms 1 and 3).
Sequence
SVAIRKRSWEEHC
Host
Goat
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 µg/ml
Product Form
Liquid
Formulation
Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Isotype Controls
Goat IgG (A121671)
Suitable Secondaries
Donkey Anti-Goat IgG H&L Antibody (AP) (A300679)
Donkey Anti-Goat IgG H&L Antibody (Biotin) (A300716)
Donkey Anti-Goat IgG H&L Antibody (FITC) (A300685)
Donkey Anti-Goat IgG H&L Antibody (HRP) (A300730)
See all Anti-Goat IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Scientific Validation Data (5)
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Figure 1: Immunohistochemistry - Anti-DLC1 (Isoforms 1 and 3) Antibody (A83189)
Anti-DLC1 Antibody (A83189) (5µg/ml) staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Figure 2: Immunofluorescence - Anti-DLC1 (Isoforms 1 and 3) Antibody (A83189)
Anti-DLC1 Antibody (A83189) - Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10µg/ml) followed by Alexa Fluor 488 secondary antibody (2µg/ml), showing nuclear and vesicle staining.
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Figure 3: Immunofluorescence - Anti-DLC1 (Isoforms 1 and 3) Antibody (A83189)
Anti-DLC1 Antibody (A83189) - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10µg/ml) followed by Alexa Fluor 488 secondary antibody (2µg/ml), showing nuclear and cytoplasmic staining.
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Figure 4: Immunohistochemistry - Anti-DLC1 (Isoforms 1 and 3) Antibody (A83189)
Anti-DLC1 Antibody (A83189) (4µg/ml) staining of paraffin embedded Mouse Adrenal Gland. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
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Figure 5: Immunohistochemistry - Anti-DLC1 (Isoforms 1 and 3) Antibody (A83189)
Anti-DLC1 Antibody (A83189) - Negative Control showing staining of paraffin embedded Mouse Adrenal Gland, with no primary antibody.
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