Anti-DLC1 Antibody (A83189)

Name: Anti-DLC1 Antibody
Brand: Antibodies.com
SKU: A83189
Price: 480.0 USD
Availability: InStock

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$480

Goat polyclonal antibody to DLC1 for ELISA, IF and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

3-5 Business Days

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Telephone

+1 (314) 370-6046

orders@antibodies.com

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Specifications

Name

Anti-DLC1 Antibody

Description

Goat polyclonal antibody to DLC1.

Applications

ELISA, IF, IHC

Dilutions

ELISA: 1:64,000, IHC: 4µg/ml, IF: 10µg/ml

Reactivity

Human, Mouse

Immunogen

Synthetic peptide corresponding to the N terminal region of human DLC1 (Isoforms 1 and 3).

Sequence

SVAIRKRSWEEHC

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Product Form

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

ARHGAP 7, ARHGAP7, Deleted in liver cancer 1, Deleted in liver cancer 1 protein, DLC 1, Dlc-1, DLC1 Rho GTPase activating protein, FLJ21120, HP, HP protein, KIAA1723, p122 RhoGAP, RHG07_HUMAN, Rho GTPase activating protein 7, Rho GTPase-activating protein 7, Rho type GTPase activating protein 7, Rho-type GTPase-activating protein 7, StAR related lipid transfer (START) domain containing 12, StAR related lipid transfer protein 12, StAR-related lipid transfer protein 12, STARD 12, StARD12, START domain containing protein 12, START domain-containing protein 12

Isotype Controls

Goat IgG (A121671)

Suitable Secondaries

See all Anti-Goat IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Immunofluorescence - Anti-DLC1 Antibody (A83189)

DLC1 (Isoforms 1 and 3) expression in U251 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-DLC1 Antibody (A83189) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Nuclear and vesicle staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 2: Immunofluorescence - Anti-DLC1 Antibody (A83189)

DLC1 (Isoforms 1 and 3) expression in U2OS cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-DLC1 Antibody (A83189) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic and nuclear staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 3: Immunohistochemistry - Anti-DLC1 Antibody (A83189)

DLC1 (Isoforms 1 and 3) expression in Mouse Adrenal Gland analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by heating in citrate buffer, pH 6. Staining was performed with Anti-DLC1 Antibody (A83189) at 4µg/ml and revealed with horseradish peroxidase (HRP).

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Figure 4: Immunohistochemistry - Anti-DLC1 Antibody (A83189)

Negative control for DLC1 (Isoforms 1 and 3) expression in Mouse Adrenal Gland analyzed by immunohistochemistry. Tissue was paraffin-embedded, and staining procedure was performed in the absence of primary antibody.

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