Western blot analysis of human 293T showing detection of 28.7 kDa DKK1 protein using Anti-DKK1 Antibody (A304872) at 1:1,000 for 2 hours at room temperature with shaking. Load: 10 µg. Block: 5% Skim Milk powder in TBST. The secondary antibody used was Goat Anti-Rabbit IgG: HRP at 1:5,000 for 1 hour at room temperature. Color Development: ECL solution for 5 minutes at room temperature. Predicted/Observed Size: 28.7 kDa.
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