Anti-DJ1 Antibody (A104338)

Immunofluorescent analysis analysis of SH-SY5Y cells stained with Anti-DJ1 Antibody (1:1,000 | red), and co-stained with Anti-Actin Antibody (1:200 | green). The blue is Hoechst staining of nuclear DNA. The Anti-DJ1 Antibody reveals strong cytoplasmic staining for DJ1 protein, while the Anti-Actin Antibody labels the submembranous actin-rich cytoskeleton, stress fibers and bundles of actin associated with cell adhesion sites.

Western blot analysis analysis of different tissue and cell lysates using Anti-DJ1 Antibody (1:2,000 | green). [1] protein standard, [2] rat brain, [3] mouse brain, [4] rat embryonic neuron-glial cells, [5] NIH-3T3, [6] HEK293, and [7] HeLa cells. Anti-DJ1 Antibody detects protein with apparent molecular weight of 21kDa in all preparations with different level of expression. The blot was simultaneously probed with mouse Anti-beta Tubulin Antibody (1;10,000 | red), revealing a single band at about 50kDa corresponding to the beta Tubulin protein.