Anti-Dishevelled 3 Antibody (A11691)

Name: Anti-Dishevelled 3 Antibody
Brand: Antibodies.com
SKU: A11691
Price: 355.0 USD
Availability: InStock

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$355

Rabbit polyclonal antibody to Dishevelled 3 for WB, IHC and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-Dishevelled 3 Antibody

Description

Rabbit polyclonal antibody to Dishevelled 3.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:20-1:50

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 580-650 of human DVL3 (NP_004414.3).

Sequence

RSGSDRRKEKDPKAGDSKSGGSGSESDHTTRSSLRGPRERAPSERSGPAASEHSHRSHHSLASSLRSHHTH

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

93 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Dishevelled-3, DSH homolog 3, DVL3, KIAA0208, Segment polarity protein dishevelled homolog DVL-3

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (5)

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Western Blot - Anti-Dishevelled 3 Antibody (A11691)

Western blot analysis of extracts of various cell lines, using Anti-Dishevelled 3 Antibody (A11691) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Immunohistochemistry - Anti-Dishevelled 3 Antibody (A11691)

Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-Dishevelled 3 Antibody (A11691) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-Dishevelled 3 Antibody (A11691)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-Dishevelled 3 Antibody (A11691) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-Dishevelled 3 Antibody (A11691)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-Dishevelled 3 Antibody (A11691) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.