Anti-DIAPH1 Antibody (A295241)

Name: Anti-DIAPH1 Antibody
Brand: Antibodies.com
SKU: A295241
Price: 475.0 USD
Availability: InStock

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$475

Rabbit polyclonal antibody to DIAPH1 for WB and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Specifications

Name

Anti-DIAPH1 Antibody

Description

Rabbit polyclonal antibody to DIAPH1.

Applications

WB, IHC

Dilutions

IHC: 1:1,000-1:5,000, WB: 1:5,000-1:15,000

Reactivity

Human

Immunogen

Synthetic peptide within amino acids 1200 and the C-terminus of human DIAPH1 (NP_005210. 1).

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Antigen affinity purification.

Concentration

1 mg/ml

Product Form

Liquid

Formulation

Supplied in Tris-Citrate/Phosphate Buffer, pH 7-8, with 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

General Notes

This antibody was affinity purified using the immunising peptide immobilized on solid support. Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1. 4 equals 1.0 mg of IgG.

Synonyms

deafness, autosomal dominant 1, DFNA1, DIA1, DIAP1, DIAP1_HUMAN, diaphanous homolog 1, Diaphanous homolog 1 (Drosophila), Diaphanous related formin 1, Diaphanous-related formin-1, DRF1, FLJ25265, hDIA1, LFHL1, low frequency hearing loss 1, p140DIA, Protein diaphanous homolog 1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-DIAPH1 Antibody (A295241)

Samples: Whole cell lysate (50 µg/lane) from untreated or siRNA treated HEK293T cells. Antibody: DIAPH1 Antibody (A295241) was used at 0.1 µg/ml. Detection: Chemiluminescence with 10 second exposure.

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Figure 2: Immunohistochemistry - Anti-DIAPH1 Antibody (A295241)

Sample: FFPE section of human breast carcinoma. Antibody: DIAPH1 Antibody (A295241) was used at a dilution of 1:1,000 ( 1µg/ml). Detection: DAB.

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Figure 3: Western Blot - Anti-DIAPH1 Antibody (A295241)

Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: DIAPH1 Antibody (A295241) was used for WB at 0.66 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.

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