Anti-DHODH Antibody (A89865)

Name: Anti-DHODH Antibody
Brand: Antibodies.com
SKU: A89865

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This product is discontinued

Anti-DHODH Antibody (A89865) has been discontinued and is no longer available.

View all Anti-DHODH Antibodies.

Specifications

Name

Anti-DHODH Antibody

Description

Rabbit polyclonal antibody to DHODH.

Applications

WB, IHC

Dilutions

WB: 1:100-1:500, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 116-395 of human DHODH (NP_001352.2).

Sequence

EIGSVTPKPQEGNPRPRVFRLPEDQAVINRYGFNSHGLSVVEHRLRARQQKQAKLTEDGLPLGVNLGKNKTSVDAAEDYAEGVRVLGPLADYLVVNVSSPNTAGLRSLQGKAELRRLLTKVLQERDGLRRVHRPAVLVKIAPDLTSQDKEDIASVVKELGIDGLIVTNTTVSRPAGLQGALRSETGGLSGKPLRDLSTQTIREMYALTQGRVPIIGVGGVSSGQDALEKIRAGASLVQLYTALTFWGPPVVGKVKRELEALLKEQGFGGVTDAIGADHRR

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

43 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

DHOdehase, Dihydroorotate dehydrogenase, Dihydroorotate dehydrogenase (quinone), Dihydroorotate dehydrogenase mitochondrial, Dihydroorotate oxidase, Human complement of yeast URA1, mitochondrial, POADS, PYRD_HUMAN, URA1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-DHODH Antibody (A89865)

Western blot analysis of various lysates, using Anti-DHODH Antibody (A89865) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 2: Immunohistochemistry - Anti-DHODH Antibody (A89865)

Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-DHODH Antibody (A89865) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-DHODH Antibody (A89865)

Immunohistochemistry analysis of paraffin-embedded human stomach using Anti-DHODH Antibody (A89865) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-DHODH Antibody (A89865)

Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-DHODH Antibody (A89865) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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