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Anti-DESI2 Antibody (A84870)

Western Blot - Anti-DESI2 Antibody (A84870) - Antibodies.com Immunohistochemistry - Anti-DESI2 Antibody (A84870) - Antibodies.com
$480
Product Datasheet

Goat polyclonal antibody to DESI2 for ELISA, WB and IHC.

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$40 to United States
Lead Time
3-5 Business Days

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+1 (314) 370-6046
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Name
Anti-DESI2 Antibody
Description
Goat polyclonal antibody to DESI2.
Applications
ELISA, WB, IHC
Dilutions
ELISA: 1:32,000, WB: 0.5-1µg/ml, IHC: 10µg/ml
Reactivity
Human
Immunogen
Synthetic peptide corresponding to the internal region of human DESI2.
Sequence
C-ELGKEYKGNAYH
Host
Goat
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 µg/ml
Molecular Weight
30 kDa
Product Form
Liquid
Formulation
Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
C1orf121, CGI 146, DESI, DeSI 2, DESI1, Desumoylating isopeptidase 2, FAM152A, family with sequence similarity 152, member A, PNAS 4, PPDE1_HUMAN, PPPDE peptidase domain containing 1, PPPDE peptidase domain-containing protein 1, PPPDE1, Protein FAM152A
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-DESI2 Antibody (A84870) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-DESI2 Antibody (A84870)
DESI2 expression in HEK293 cell lysate analyzed by western blot. Cells were transfected to overexpress MYC tagged DESI2 an lysed in RIPA buffer. Primary incubation was performed for 1 hour on 10µg protein per lane with Anti-DESI2 Antibody (A84870) at 0.5µg/ml (Lane A) or Anti-MYC at 1:5000 dilution (Lane C) and detected with chemiluminescence. Control: Mock-transfected HEK293 cells incubated with Anti-DESI2 Antibody (A84870) at 1mg/ml (Lane B).
Immunohistochemistry - Anti-DESI2 Antibody (A84870) - Antibodies.comEnlarge Image
Figure 2: Immunohistochemistry - Anti-DESI2 Antibody (A84870)
DESI2 expression in Human Skin analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-DESI2 Antibody (A84870) at 10µg/ml and revealed with alkaline phosphatase (AP).
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