Anti-Dengue Virus NS1 Glycoprotein Matched Antibody Pair (A58136)

Anti-Dengue Virus NS1 Glycoprotein Matched Antibody Pair

Lateral Flow Assay (LFA)

Unconjugated

95% as determined by SDS-PAGE (coomassie staining).

3.6mg/ml

Dengue NS1 Gold Conjugation Antibody: Supplied in Phosphate Buffered Saline with Sodium Nitrate, Dengue NS1 Membrane Coating Antibody: Supplied in Phosphate Buffered Saline with Sodium Nitrate.

Shipped at 4°C. Short term (2-4 weeks) store at 4°C. Long term store at -20°C.

1. 1-2 days fresh prepared gold particle with maximal homogeneity in gold particle size, ? 525-529, OD 1.2-1.4. 2. Conjugation at pH 8.5, conjugation time should be 6-8 hours. 3. Blocking condition: remove supernatant and re-suspend gold pellet bound with anti-dengue NS1 to OD 8.0-8.2, add casein to the final 0.3-0.4%. 4. Treat sample pad with 50 mM sodium borate, 0.5% mouse serum and 0.5% Triton X-100, pH 8.