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Anti-XPA Antikörper [5F12] (A355)

Dieses Produkt ist identisch mit Abcam (ab65963). Entdecken Sie mehr für weniger.

Western blot of endogenous XPA protein. Hela cell whole extract (20µg). Anti-XPA Antibody was used at 1:2,000 dilution. Goat Anti-Mouse IgG Antibody (HRP) was used as a secondary at 1:20,000 dilution. Western blot of XPA. Lane 1. Extract of Hela cells (XPA wild type). Lane 2. Extract of XP12ROSV cells (XPA deficient). The primary and the secondary antibodies were used at 1:2,000 and 1:20,000 dilutions. Immunofluorescence staining of human fibroblast cells (GM0637) using Anti-XPA Antibody. The cells were non-irradiated (left) or irradiated with UV at 20 J/m2 and fixed after 30 min with paraformaldehyde. Anti-XPA Antibody was used at 1:100 dilution and Goat Anti-Mouse IgG (Alexa 448) was used as a secondary at 1:5,000 dilution.
Knockout-validierter Antikörper
$535

Monoklonal [5F12] Maus Antikörper gegen XPA für WB, IF, ELISA und Inhibition Assay.

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Versandkosten
$40
Vorlaufzeit
Lieferung in 4-6 Werktagen

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Produktname
Anti-XPA Antibody [5F12]
Beschreibung
Mouse monoclonal (5F12) antibody to XPA.
Anwendungen
WB, IF, ELISA, Inhibition Assay
Verdünnungen
WB: 1:1,000-1:10,000, IF: 1:100-1:1,000
Reaktivität
Human
Immunogen
Recombinant full length human XPA protein.
Epitop
Amino acids 30-47.
Wirt
Mouse
Klonalität
Monoclonal
Klon
5F12
Isotyp
IgG2b
Konjugat

Unconjugated

Reinigung
Ion exchange chromatography.
Konzentration
1 mg/ml
Produktform
Liquid
Formulierung
Supplied in Phosphate Buffered Saline, pH 7.2, with 50% Glycerol (filter sterilised, without Sodium Azide, and carrier free).
Lagerung
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyme
DNA repair protein complementing XP A cells, DNA repair protein complementing XP-A cells, DNA repair protein complementing XPA cells, Excision repair controlling, Xeroderma pigmentosum 1, Xeroderma pigmentosum complementation group A, Xeroderma pigmentosum group A complementing protein, Xeroderma pigmentosum group A-complementing protein, XP 1, XP1, Xpac, XPA_HUMAN
Isotyp-Kontrollantikörper
Kompatible Sekundärantikörper
Haftungsausschluss
Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.
Western blot of endogenous XPA protein. Hela cell whole extract (20µg). Anti-XPA Antibody was used at 1:2,000 dilution. Goat Anti-Mouse IgG Antibody (HRP) was used as a secondary at 1:20,000 dilution.Bild Vergrößern
Figure 1: Anti-XPA Antibody [5F12] (70-031)
Anti-XPA Antibody
Western blot of XPA. Lane 1. Extract of Hela cells (XPA wild type). Lane 2. Extract of XP12ROSV cells (XPA deficient). The primary and the secondary antibodies were used at 1:2,000 and 1:20,000 dilutions.Bild Vergrößern
Figure 2: Anti-XPA Antibody [5F12] (70-031)
Anti-XPA Antibody
Immunofluorescence staining of human fibroblast cells (GM0637) using Anti-XPA Antibody. The cells were non-irradiated (left) or irradiated with UV at 20 J/m2 and fixed after 30 min with paraformaldehyde. Anti-XPA Antibody was used at 1:100 dilution and Goat Anti-Mouse IgG (Alexa 448) was used as a secondary at 1:5,000 dilution.Bild Vergrößern
Figure 3: Immunofluorescence - Anti-XPA Antibody [5F12] (70-031)
Anti-XPA Antibody

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