Synthetic peptide corresponding to the internal region of human Wnt3.
Sequenz
CGRGHNTRTEKRKEK
Wirt
Goat
Klonalität
Polyclonal
Isotyp
IgG
Konjugat
Unconjugated
Reinigung
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Konzentration
500 µg/ml
Molekulargewicht
40 kDa
Geschätztes MW
39.6kDa
Produktform
Liquid
Formulierung
Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.
Lagerung
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyme
Int 4, INT4, MGC131950, MGC138321, MGC138323, Proto-oncogene Int-4 homolog, Proto-oncogene Wnt-3, wingless type MMTV integration site family member 3, Wnt 3 proto oncogene protein, WNT 3 proto oncogene protein precursor, WNT3_HUMAN
Figure 1: Western Blot - Anti-Wnt3 Antibody (A84213)
Wnt3 expression in HEK293 cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-Wnt3 Antibody (A84213) at 1µg/ml and detected by chemiluminescence.
Wnt3 expression in Human Breast analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-Wnt3 Antibody (A84213) at 2µg/ml and revealed with horseradish peroxidase (HRP).
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