Anti-WISP1 Antikörper (A82877)

Name: Anti-WISP1 Antibody
Brand: Antibodies.com
SKU: A82877
Price: 480.0 USD
Availability: InStock

Dieses Produkt ist identisch mit Novus (NB100-77339). Entdecken Sie mehr für weniger.

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$480

Polyklonal Ziege Antikörper gegen WISP1 für ELISA, WB, IF und Flow Cytometry.

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Versand

Versandkosten

$40

Vorlaufzeit

Lieferung in 3-5 Werktagen

Kontakt

Telefon

+1 (314) 370-6046

orders@antibodies.com

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Produktdetails

Produktname

Anti-WISP1 Antibody

Beschreibung

Goat polyclonal antibody to WISP1.

Anwendungen

ELISA, WB, IF, Flow Cytometry

Verdünnungen

ELISA: 1:128,000, WB: 0.1-0.3µg/ml, IF: 10µg/ml, Flow Cytometry: 10µg/ml

Reaktivität

Human

Immunogen

Synthetic peptide corresponding to the C terminal region of human WISP1.

Sequenz

C-ESYPDFSEIAN

Wirt

Goat

Klonalität

Polyclonal

Isotyp

IgG

Konjugat

Unconjugated

Reinigung

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Konzentration

500 µg/ml

Molekulargewicht

32-35 kDa

Geschätztes MW

30.7kDa

Produktform

Liquid

Formulierung

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Lagerung

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyme

CCN 4, CCN family member 4, CCN4, WISP 1, WISP 1c, WISP 1i, WISP 1tc, WISP1c, WISP1i, WISP1tc, Wnt 1 induced secreted protein, WNT 1 induced secreted protein 1, WNT 1 inducible signaling pathway protein 1, Wnt 1 signaling pathway protein 1, Wnt-1 inducible signaling pathway protein 1, Wnt1 induced secreted protein, WNT1 induced secreted protein 1, WNT1 inducible signaling pathway protein 1, Wnt1 signaling pathway protein 1, WNT1-inducible-signaling pathway protein 1

Isotyp-Kontrollantikörper

Goat IgG (A121671)

Kompatible Sekundärantikörper

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

Produktbilder (5)

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Figure 1: Western Blot - Anti-WISP1 Antibody (A82877)

WISP1 expression in Human Tonsil lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-WISP1 Antibody (A82877) at 0.1µg/ml and detected by chemiluminescence.

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Figure 2: Western Blot - Anti-WISP1 Antibody (A82877)

WISP1 expression in Caco-2 cell lysate 1 (A) and Caco-2 cell lysate 2 (B) + petide (C) analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-WISP1 Antibody (A82877) at 0.3µg/ml and detected by chemiluminescence.

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Figure 3: Immunofluorescence - Anti-WISP1 Antibody (A82877)

WISP1 expression in A431 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-WISP1 Antibody (A82877) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 4: Immunofluorescence - Anti-WISP1 Antibody (A82877)

WISP1 expression in U2OS cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-WISP1 Antibody (A82877) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.