Rabbit polyclonal antibody to WAVE1 (Phospho-Tyr125)
Specificity
The antibody detects endogenous levels of WAVE1 only when phosphorylated at tyrosine 125.
Applications
WB, IHC
Reactivity
Human, Mouse
Immunogen
Peptide sequence around phosphorylation site of tyrosine 125 (E-T-Y(p)-D-V) derived from Human WAVE1.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20˚C
Synonyms
Actin-binding protein WASF1, KIAA0269, Protein WAVE-1, SCAR1, Verprolin homology domain-containing protein 1, WASF1, WASP family protein member 1, Wiskott-Aldrich syndrome protein family member 1
Western blot analysis of extracts from NIH/3T3 cells, treated with Insulin (0.01U/ml, 15mins), using WAVE1 (Phospho-Tyr125) antibody #12156. The lane on the right is treated with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using WAVE1 (Phospho-Tyr125) antibody #12156. The picture on the right is treated with the synthesized peptide.
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