Anti-VPS35 Antikörper (A83699)

Name: Anti-VPS35 Antibody
Brand: Antibodies.com
SKU: A83699
Price: 480.0 USD
Availability: InStock

Dieses Produkt ist identisch mit Abcam (ab10099). Entdecken Sie mehr für weniger.

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$480

Polyklonal Ziege Antikörper gegen VPS35 für ELISA, WB, IHC und IF.

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Versand

Versandkosten

$40

Vorlaufzeit

Lieferung in 3-5 Werktagen

Kontakt

Telefon

+1 (314) 370-6046

orders@antibodies.com

Garantien

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Produktdetails

Produktname

Anti-VPS35 Antibody

Beschreibung

Goat polyclonal antibody to VPS35.

Anwendungen

ELISA, WB, IHC, IF

Verdünnungen

ELISA: 1:128,000, WB: 0.03-0.1µg/ml, IHC: 8µg/ml, IF: 10µg/ml

Reaktivität

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to the C terminal region of human VPS35.

Sequenz

C-SPESEGPIYEGLIL

Wirt

Goat

Klonalität

Polyclonal

Isotyp

IgG

Konjugat

Unconjugated

Reinigung

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Konzentration

500 µg/ml

Molekulargewicht

90 kDa

Geschätztes MW

91.7kDa

Produktform

Liquid

Formulierung

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Lagerung

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyme

DKFZp434E1211, DKFZp434P1672, FLJ10752, FLJ13588, FLJ20388, hVPS35, Maternal embryonic 3, Maternal-embryonic 3, MEM 3, MEM3, PARK17, TCCCTA00141, Vacuolar protein sorting 35, Vacuolar protein sorting 35 (yeast), Vacuolar protein sorting 35 homolog, Vacuolar protein sorting associated protein 35, Vacuolar protein sorting-associated protein 35, Vesicle protein sorting 35, VPS 35, VPS35_HUMAN

Isotyp-Kontrollantikörper

Goat IgG (A121671)

Kompatible Sekundärantikörper

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

Produktbilder (6)

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Figure 1: Western Blot - Anti-VPS35 Antibody (A83699)

VPS35 expression in Human (A) Mouse (B) and Rat (C) Brain lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-VPS35 Antibody (A83699) at 0.03µg/ml and detected by chemiluminescence.

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Figure 2: Western Blot - Anti-VPS35 Antibody (A83699)

VPS35 expression in HepG2 (A) and HEK293 (B) cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-VPS35 Antibody (A83699) at 0.03µg/ml and detected by chemiluminescence.

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Figure 3: Immunofluorescence - Anti-VPS35 Antibody (A83699)

VPS35 expression in HEK293 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-VPS35 Antibody (A83699) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic/vesicle staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 4: Immunofluorescence - Anti-VPS35 Antibody (A83699)

VPS35 expression in U2OS cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-VPS35 Antibody (A83699) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic/vesicle staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.