Anti-VPS35 Antikörper [5A9] (A305033)

Name: Anti-VPS35 Antibody [5A9]
Brand: Antibodies.com
SKU: A305033
Price: 580.0 USD
Availability: InStock

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$580

Monoklonal [5A9] Maus Antikörper gegen VPS35 für WB, ICC/IF und IP.

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Versand

Versandkosten

$40

Vorlaufzeit

Lieferung in 5-8 Werktagen

Kontakt

Telefon

+1 (314) 370-6046

orders@antibodies.com

Garantien

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Produktdetails

Produktname

Anti-VPS35 Antibody [5A9]

Beschreibung

Mouse monoclonal [5A9] antibody to VPS35.

Anwendungen

WB, ICC/IF, IP

Verdünnungen

WB: 1:1,000, ICC/IF: 1:200, IP: 1:200

Reaktivität

Human, Mouse, Rat

Immunogen

Full length recombinant human VSP35.

Wirt

Mouse

Klonalität

Monoclonal

Klon

5A9

Isotyp

IgG1

Konjugat

Unconjugated

Reinigung

Protein G purification.

Konzentration

1 mg/ml

Molekulargewicht

~92 kDa

Produktform

Liquid

Formulierung

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.

Lagerung

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyme

DKFZp434E1211, DKFZp434P1672, FLJ10752, FLJ13588, FLJ20388, hVPS35, Maternal embryonic 3, Maternal-embryonic 3, MEM 3, MEM3, PARK17, TCCCTA00141, Vacuolar protein sorting 35, Vacuolar protein sorting 35 (yeast), Vacuolar protein sorting 35 homolog, Vacuolar protein sorting associated protein 35, Vacuolar protein sorting-associated protein 35, Vesicle protein sorting 35, VPS 35, VPS35_HUMAN

Isotyp-Kontrollantikörper

Kompatible Sekundärantikörper

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

Produktbilder (8)

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Figure 1: Immunofluorescence - Anti-VPS35 Antibody [5A9] (A305033)

Immunocytochemistry/Immunofluorescence analysis of human A549 WT, VPS35 KO cells, using Anti-VPS35 Antibody [5A9] (A305033). The secondary antibody used was Donkey Anti-Mouse AlexaFluor 594. Clone can detect VPS35 at 1/2000 concentration.

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Figure 2: Immunoprecipitation - Anti-VPS35 Antibody [5A9] (A305033)

Immunoprecipitation analysis of mouse embryonic fibroblast using Anti-VPS35 Antibody [5A9] (A305033). Three amounts ofA305033 (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4°C and next incubated with 250ug of A549 lysate for 2 hours at 4°C.

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Figure 3: Immunoprecipitation - Anti-VPS35 Antibody [5A9] (A305033)

Immunoprecipitation analysis of mouse embryonic fibroblast using Anti-VPS35 Antibody [5A9] (A305033). Three amounts of A305033 (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4°C and next incubated with 250ug of MEF lysate for 2 hours at 4°C.

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Figure 4: Immunoprecipitation - Anti-VPS35 Antibody [5A9] (A305033)

Immunoprecipitation analysis of mouse embryonic fibroblast using Anti-VPS35 Antibody [5A9] (A305033). 500 µL cell culture supernatants were incubated with 10 µL of Protein A/G resin beads for 1 hour at 4°C. A305033 clone 5A9 depletes VPS35 from the A549 cell extract..