Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
hVps34, Phosphatidylinositol 3-kinase catalytic subunit type 3, Phosphatidylinositol 3-kinase p100 subunit, Phosphoinositide-3-kinase class 3, PI3-kinase type 3, PI3K type 3, PIK3C3, PtdIns-3-kinase type 3
Western Blot - Anti-VPS34 Antibody [ARC0286] (A306019)
Western blot analysis of extracts of various cell lines, using Anti-VPS34 Antibody [ARC0286] (A306019) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Immunofluorescence analysis of NIH-3T3 cells using Anti-VPS34 Antibody [ARC0286] (A306019) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Western Blot - Anti-VPS34 Antibody [ARC0286] (A306019)
Immunoprecipitation analysis of 600µg extracts of rat brain cells using 3µg of Anti-VPS34 Antibody [ARC0286] (A306019). This Western blot was performed on the immunoprecipitate using Anti-VPS34 Antibody [ARC0286] (A306019) at a dilution of 1:500.
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