This antibody recognises human vinculin, also known as metavinculin. Vinculin is a highly conserved, cytoskeletal protein associated with cell-cell and cell-matrix adhesion. Vinculin is an actin filament (F-actin)-binding protein involved in anchoring F-actin to the membrane. Mouse anti Human vinculin antibody, clone V284 detects vinculin as a ~130 kDa band in homogenates of platelets and other cells. It reacts with leucocytes, muscle cells, epithelial cells and fibroblasts.
Anwendungen
WB, IHC-P, IP, Flow Cytometry, IHC-Fr, IF
Verdünnungen
WB: 1:500 - 1:2,000, Use 10µl of the suggested working dilution to label 1x106 cells in 100µl
Reaktivität
Human, Rat, Rabbit, Chicken, Mouse
Immunogen
Purified vinculin from human platelets.
Wirt
Mouse
Klonalität
Monoclonal
Klon
V284
Isotyp
IgG1
Konjugat
Unconjugated
Reinigung
Protein A affinity chromatography of tissue culture supernatant.
Konzentration
1 mg/ml
Produktform
Liquid
Formulierung
Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.
Lagerung
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
Allgemeine Hinweise
Mouse anti Human vinculin antibody, clone V284 recognizes human vinculin, also known as metavinculin. Vinculin is a highly conserved, cytoskeletal protein associated with cell-cell and cell-matrix adhesion. Vinculin is an actin filament (F-actin)-binding protein involved in anchoring F-actin to the membrane.Mouse anti Human vinculin antibody, clone V284 detects vinculin as a ~130 kDa band in homogenates of platelets and other cells. It reacts with leucocytes, muscle cells, epithelial cells and fibroblasts.