Anti-Vinculin Antikörper [ARC51900] (A305534)

Name: Anti-Vinculin Antibody [ARC51900]
Brand: Antibodies.com
SKU: A305534
Price: 470.0 USD
Availability: InStock

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$470

Monoklonal [ARC51900] Kaninchen Antikörper gegen Vinculin für WB, ICC/IF und IP.

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Versand

Versandkosten

$40

Vorlaufzeit

Lieferung in 5-8 Werktagen

Kontakt

Telefon

+1 (314) 370-6046

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Produktdetails

Produktname

Anti-Vinculin Antibody [ARC51900]

Beschreibung

Rabbit monoclonal [ARC51900] antibody to Vinculin.

Anwendungen

WB, ICC/IF, IP

Verdünnungen

WB: 1:10,000-1:30,000, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000

Reaktivität

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 800-900 of human Vinculin (NP_054706.1).

Sequenz

DAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMM

Wirt

Rabbit

Klonalität

Monoclonal

Klon

ARC51900

Isotyp

IgG

Konjugat

Unconjugated

Reinigung

Affinity purification.

Molekulargewicht

124 kDa

Produktform

Liquid

Formulierung

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Lagerung

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyme

CMD1W, CMH15, Epididymis luminal protein 114, HEL114, Metavinculin, MV, MVCL, OTTHUMP00000019861, OTTHUMP00000019862, VCL, VINC, VINC_HUMAN

Isotyp-Kontrollantikörper

Kompatible Sekundärantikörper

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

Produktbilder (3)

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Figure 1: Western Blot - Anti-Vinculin Antibody [ARC51900] (A305534)

Western blot analysis of various lysates, using Anti-Vinculin Antibody [ARC51900] (A305534) at 1:250,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 2: Immunofluorescence - Anti-Vinculin Antibody [ARC51900] (A305534)

Confocal imaging of HeLa cells using Anti-Vinculin Antibody [ARC51900] (A305534), at a dilution of 1:100, (red). DAPI was used for nuclear staining (Blue). Objective: 60x.

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Figure 3: Western Blot - Anti-Vinculin Antibody [ARC51900] (A305534)

Immunoprecipitation analysis of 300µg extracts of HeLa cells using 3µg of Anti-Vinculin Antibody [ARC51900] (A305534). This Western blot was performed on the immunoprecipitate using Anti-Vinculin Antibody [ARC51900] (A305534) at a dilution of 1:1000.