Recombinant rabbit monoclonal [VIL1/8105R] antibody to Villin.
Spezifität
Recognizes a protein of 95kDa, which is identified as villin. It is a major constituent in the microvilli, which compose the brush border of epithelial cells forming absorptive surfaces of the intestinal and renal proximal tubular epithelia. Anti-Villin labels the brush border area in the gastrointestinal mucosal epithelium and urogenital tract. Among neoplasms, villin is predominantly expressed in tumors of colorectal origin.Antibody to villin is useful in identifying malignant cells from primary and metastatic colorectal carcinomas. This antibody also labels Merkel cells of the skin.
Anwendungen
IHC-P
Positivkontrolle
A549, HepG2 or HCT116 cells. Human colon or rectum.
Verdünnungen
IHC: 1-2 µg/ml
Reaktivität
Human
Immunogen
Recombinant fragment (around Amino Acids 600-700) of human Villin protein. The exact sequence is proprietary.
Wirt
Rabbit
Klonalität
Monoclonal
Klon
VIL1/8105R
Isotyp
IgG
Leichte Ketten
kappa
Konjugat
Unconjugated
Reinigung
Protein A/G chromatography.
Konzentration
200 µg/ml
Molekulargewicht
93 kDa
Produktform
Liquid
Formulierung
Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.
Lagerung
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.