Anti-UCHL1 Antibody [BH7] (A85351)

Immunofluorescent analysis of rat hippocampal section stained with Anti-UCH-L1 Antibody [BH7] (A85351), dilution 1:5,000, in green and co-stained with Anti-Fox3 Antibody (A85403), dilution 1:2,000, in red. The blue is Hoechst staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, the brain was post-fixed for 24 hours, cut to 45 µM, and free-floating sections were stained with the above antibodies. The UCH-L1 antibody stains the cell body and dendrites of hippocampal neurons, while the FOX3 antibody labels nuclei of the neuronal cells.

Western blot analysis of tissue lysates using Anti-UCH-L1 Antibody [BH7] (A85351), dilution 1:10,000, in green: [Lane 1] protein standard (red), [Lane 2] rat brain, [Lane 3] rat spinal cord, [Lane 4] mouse brain, [Lane 5] mouse spinal cord, [Lane 6] pig brain, and [Lane 7] pig spinal cord. The single band at 24 kDa corresponds to the UCH-L1 protein.

Immunofluorescent analysis of cortical neuron-glial culture from E20 rat stained with Anti-UCHL1 Antibody [BH7] (A85351) at a dilution of 1:5,000 (green) and costained with Anti-GFAP Antibody (A85307) at a dilution of 1:5,000 (red). Nuclei were stained with DAPI (blue). The Anti-UCHL1 Antibody [BH7] (A85351) antibody stains cell bodies and dendrites of neurons, while the GFAP antibody labels astrocytes.

A section of rat spinal cord stained with Anti-UCHL1 Antibody (red) and Anti-NF-H Antibody (A85336 | green). The large cells are a-motorneurons and UCHL1 fills the cytoplasm of their perikarya and dendrites. The Anti-NF-H Antibody binds primarily to phosphorylated axonal forms of NF-H, and so stains axons coursing between the large motor neurons.