Immunocytochemistry/Immunofluorescence analysis of human cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-Ubiquitin Antibody [6C11-B3] (A305079), at 1:100 for 12 hours at 4°C. The secondary antibody used was FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Diffuse nuclear and cytoplasmic staining. Magnification: 20x.(A) DAPI (blue) nuclear stain. (B) Anti-Ubiquitin Antibody. (C) Composite.
Figure 2: Western Blot - Anti-Ubiquitin Antibody [6C11-B3] (A305079)
Western blot analysis of human Cell lysates showing detection of Ubiquitin protein using Anti-Ubiquitin Antibody [6C11-B3] (A305079) at 1:1,000 for 2 hours at room temperature. Load: 15 µg. Block: 1.5% BSA for 30 minutes at room temperature. The secondary antibody used was Sheep Anti-Mouse IgG: HRP for 1 hour at room temperature.
Immunocytochemistry/Immunofluorescence analysis of human cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-Ubiquitin Antibody [6C11-B3] (A305079), at 1:100 for 12 hours at 4°C. The secondary antibody used was APC Goat Anti-Mouse (red) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Diffuse nuclear and cytoplasmic staining. Magnification: 100x.(A) DAPI (blue) nuclear stain. (B) Anti-Ubiquitin Antibody. (C) Composite.
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