Immunofluorescent analysis of rat brain section stained with Anti-Tyrosine Hydroxylase Antibody (1:1,000 | red). The blue is Hoechst staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45µM, and free-floating sections were stained with the above antibodies. Anti-Tyrosine Hydroxylase Antibody stains TH expressing neuronal processes, which are particularly numerous in the striatum, at the right of the image.
Figure 2: Western Blot - Anti-Tyrosine Hydroxylase Antibody [4H2] (A104315)
Western blot analysis of tissue and cell lysates using Anti-Tyrosine Hydroxylase Antibody (1:5,000 | green): [1] protein standard (red), [2] rat brain caudate/putmen and [3] PC12 cells. The strong band at about 58kDa corresponds to TH protein. The weak band at about 80kDa in the rat brain homogenate is of unknown origin, but does not affect the specific cell and process staining of this antibody.
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