Anti-TRIM29 Antikörper (A84758)

Name: Anti-TRIM29 Antibody
Brand: Antibodies.com
SKU: A84758
Price: 480.0 USD
Availability: InStock

Dieses Produkt ist identisch mit Abcam (ab99486). Entdecken Sie mehr für weniger.

Bild Vergrößern

$480

Polyklonal Ziege Antikörper gegen TRIM29 für ELISA, WB, IF, Flow Cytometry und IHC.

100% Qualitätsgarantie

Preis-Match-Garantie

Versand

Versandkosten

$40

Vorlaufzeit

Lieferung in 3-5 Werktagen

Kontakt

Telefon

+1 (314) 370-6046

orders@antibodies.com

Garantien

100% Qualitätsgarantie

Preis-Match-Garantie

Produktdetails

Produktname

Anti-TRIM29 Antibody

Beschreibung

Goat polyclonal antibody to TRIM29.

Anwendungen

ELISA, WB, IF, Flow Cytometry, IHC

Verdünnungen

ELISA: 1:128,000, WB: 0.1-1µg/ml, IHC: 3.75µg/ml, IF: 10µg/ml

Reaktivität

Human

Immunogen

Synthetic peptide corresponding to the internal region of human TRIM29.

Sequenz

C-EKDRIKSFTTNE

Wirt

Goat

Klonalität

Polyclonal

Isotyp

IgG

Konjugat

Unconjugated

Reinigung

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Konzentration

500 µg/ml

Molekulargewicht

37 kDa

Geschätztes MW

37.3kDa

Produktform

Liquid

Formulierung

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Lagerung

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyme

Ataxia telangiectasia group D associated protein, Ataxia telangiectasia group D-associated protein, ATDC, FLJ36085, TRI29_HUMAN, TRIM 29, Tripartite motif containing 29, Tripartite motif containing protein 29, Tripartite motif protein 29, Tripartite motif protein TRIM29, Tripartite motif-containing protein 29

Isotyp-Kontrollantikörper

Goat IgG (A121671)

Kompatible Sekundärantikörper

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

Produktbilder (5)

Bild Vergrößern

Figure 1: Western Blot - Anti-TRIM29 Antibody (A84758)

TRIM29 expression in Peripheral Blood Mononucleocyte (A) and HeLa (B) cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary incubation was performed with Anti-TRIM29 Antibody (A84758) at 1µg/ml (A) or 0.1 µg/ml (B) and detected by chemiluminescence.

Bild Vergrößern

Figure 2: Immunofluorescence - Anti-TRIM29 Antibody (A84758)

TRIM29 expression in A431 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-TRIM29 Antibody (A84758) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 4µg/ml. Cytoplasmic and intermediate filament staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 4µg/ml.

Bild Vergrößern

Figure 3: Immunofluorescence - Anti-TRIM29 Antibody (A84758)

TRIM29 expression in U2OS cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-TRIM29 Antibody (A84758) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 4µg/ml. Nuclear staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 4µg/ml.

Bild Vergrößern

Figure 4: Flow Cytometry - Anti-TRIM29 Antibody (A84758)

TRIM29 expression in A431 cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-TRIM29 Antibody (A84758) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.