Mouse monoclonal [MEM-75] antibody to Transferrin Receptor (Biotin).
Spezifität
This antibody reacts with an extracellular epitope of CD71 antigen, a 95 kDa type II homodimeric transmembrane glycoprotein expressed on activated B and T lymphocytes, macrophages, and erythroid precursors. It is lost on resting blood leukocytes. This antibody does not block binding of transferrin to the receptor.
Anwendungen
Flow Cytometry, IP, ICC
Verdünnungen
Flow Cytometry: 2-5 µg/ml.
Reaktivität
Human
Immunogen
NALM-6 human pre-B cell line.
Wirt
Mouse
Klonalität
Monoclonal
Klon
MEM-75
Isotyp
IgG1
Konjugat
Biotin
Reinigung
This antibody is conjugated with Biotin LC-NHS ester under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free Biotin.
Konzentration
1 mg/ml
Geschätztes MW
95 kDa
Produktform
Liquid
Formulierung
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Flow cytometric analysis of human PHA stimulated peripheral blood mononuclear cells stained with Anti-CD71 Antibody [MEM-75] (Biotin) (concentration in sample is 0.6 µg/ml, Streptavidin-APC).
Separation of cells stained with Anti-CD71 Antibody [MEM-75] (Biotin) (concentration in sample is 0.6 µg/ml, Streptavidin-APC, red-filled) from cells unstained by primary antibody (Streptavidin-APC, black-dashed) in flow cytometry analysis (surface staining).