This antibody recognises human tumor necrosis factor ligand superfamily member 10 otherwise known as TRAIL, CD253 or Apo2L. CD253 is a 281 amino acid type II transmembrane glycoprotein and member of the tumour necrosis factor superfamily, which induces apoptosis in tumour and transformed cells, but not normal cells.Activation of apoptosis by CD253 occurs through binding to the death-signaling receptors DR4 (TRAILR1) and DR5 (TRAILR2), leading to formation of the death-inducing signaling complex (DISC) and the triggering of a proteolytic caspase cascade. There are however decoy receptors known as DcR1 (TRAILR3) and DcR2 (TRAILR4) which competitively bind to CD253 and are unable to initiate apoptosis.CD253 also plays a role in immune surveillance, acting as a mediator of monocyte, NK cell, dendritic cell and T cell anti-tumour and anti-viral cytotoxicity.

Anwendungen

WB, ELISA, IHC-P, Functional Assays

Verdünnungen

ELISA: 0.5 - 2 µg/ml, Functional Assays: 150ng/ml, IHC-P: 0.25 µg/ml, WB: 0.1 - 0.2 µg/ml

Reaktivität

Human

Immunogen

Recombinant human CD253.

Wirt

Rabbit

Klonalität

Polyclonal

Isotyp

IgG

Konjugat

Unconjugated

Konzentration

1 mg/ml after reconstitution.

Produktform

Lyophilized

Rekonstitution

Reconstitute with 0.1 ml of distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Gently mix after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. For functional studies do not add sodium azide

Formulierung

Supplied in Phosphate Buffered Saline.

Lagerung

Lyophilized: Store at -20°C. Reconstituted: Store undiluted at -20°C, avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

Allgemeine Hinweise

Rabbit anti Human CD253 antibody recognizes human tumor necrosis factor ligand superfamily member 10 otherwise known as TRAIL, CD253 or Apo2L. CD253 is a 281 amino acid type II transmembrane glycoprotein and member of the tumour necrosis factor superfamily, which induces apoptosis in tumour and transformed cells, but not normal cells.Activation of apoptosis by CD253 occurs through binding to the death-signaling receptors DR4 (TRAILR1) and DR5 (TRAILR2), leading to formation of the death-inducing signaling complex (DISC) and the triggering of a proteolytic caspase cascade. There are however decoy receptors known as DcR1 (TRAILR3) and DcR2 (TRAILR4) which competitively bind to CD253 and are unable to initiate apoptosis.CD253 also plays a role in immune surveillance, acting as a mediator of monocyte, NK cell, dendritic cell and T cell anti-tumour and anti-viral cytotoxicity.

Synonyme

Apo 2 ligand, APO 2L, Apo-2 ligand, Apo-2L, APO2L, CD253, CD253 antigen, Chemokine tumor necrosis factor ligand superfamily member 10, Protein TRAIL, TL2, TNF Related Apoptosis Inducing Ligand, TNF related apoptosis inducing ligand TRAIL, TNF-related apoptosis-inducing ligand, TNF10_HUMAN, TNFSF10, Tumor necrosis factor (ligand) family member 10, Tumor Necrosis Factor (ligand) Superfamily Member 10, Tumor necrosis factor apoptosis inducing ligand splice variant delta, Tumor necrosis factor ligand superfamily member 10

Isotyp-Kontrollantikörper

Kompatible Sekundärantikörper

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.