Anti-TOMM20 Antikörper (A307960)

Name: Anti-TOMM20 Antibody
Brand: Antibodies.com
SKU: A307960
Price: 470.0 USD
Availability: InStock

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$470

Polyklonal Kaninchen Antikörper gegen TOMM20 für WB, IHC und ICC/IF.

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Versandkosten

$40

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Lieferung in 5-8 Werktagen

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+1 (314) 370-6046

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Stichworte:

Mitochondrial Marker

Produktdetails

Produktname

Anti-TOMM20 Antibody

Beschreibung

Rabbit polyclonal antibody to TOMM20.

Anwendungen

WB, IHC, ICC/IF

Verdünnungen

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reaktivität

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-145 of human TOM20 (NP_055580.1).

Sequenz

MVGRNSAIAAGVCGALFIGYCIYFDRKRRSDPNFKNRLRERRKKQKLAKERAGLSKLPDLKDAEAVQKFFLEEIQLGEELLAQGEYEKGVDHLTNAIAVCGQPQQLLQVLQQTLPPPVFQMLLTKLPTISQRIVSAQSLAEDDVE

Wirt

Rabbit

Klonalität

Polyclonal

Isotyp

IgG

Konjugat

Unconjugated

Reinigung

Affinity purification.

Molekulargewicht

16 kDa

Produktform

Liquid

Formulierung

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Lagerung

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyme

KIAA0016, MAS20, MGC117367, Mitochondrial 20 kDa outer membrane protein, Mitochondrial import receptor subunit TOM20 homolog, MOM19, Outer mitochondrial membrane receptor Tom20, TOM20, TOM20_HUMAN, Translocase of outer mitochondrial membrane 20 homolog (yeast), Translocase of outer mitochondrial membrane 20 homolog type II

Isotyp-Kontrollantikörper

Kompatible Sekundärantikörper

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

Produktbilder (11)

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Figure 1: Western Blot - Anti-TOMM20 Antibody (A307960)

Western blot analysis of extracts of various cell lines, using Anti-TOMM20 Antibody (A307960) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Western Blot - Anti-TOMM20 Antibody (A307960)

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Figure 3: Western Blot - Anti-TOMM20 Antibody (A307960)

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Figure 4: Western Blot - Anti-TOMM20 Antibody (A307960)

Western blot analysis of extracts from wild type (WT) and [KD Validated] TOM20 knockdown (KD) 293T cells, using Anti-TOMM20 Antibody (A307960) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.

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Figure 5: Immunohistochemistry - Anti-TOMM20 Antibody (A307960)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-TOMM20 Antibody (A307960) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 6: Immunohistochemistry - Anti-TOMM20 Antibody (A307960)

Immunohistochemistry analysis of paraffin-embedded mouse spleen using Anti-TOMM20 Antibody (A307960) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 7: Immunohistochemistry - Anti-TOMM20 Antibody (A307960)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-TOMM20 Antibody (A307960) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 8: Immunohistochemistry - Anti-TOMM20 Antibody (A307960)

Immunohistochemistry analysis of paraffin-embedded rat kidney using Anti-TOMM20 Antibody (A307960) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 9: Immunofluorescence - Anti-TOMM20 Antibody (A307960)

Immunofluorescence analysis of C6 cells using Anti-TOMM20 Antibody (A307960) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 10: Immunofluorescence - Anti-TOMM20 Antibody (A307960)

Immunofluorescence analysis of NIH-3T3 cells using Anti-TOMM20 Antibody (A307960) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 11: Immunofluorescence - Anti-TOMM20 Antibody (A307960)

Immunofluorescence analysis of U-2 OS cells using Anti-TOMM20 Antibody (A307960) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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