Rekombinant Anti-TIM 3 Antikörper [DM83] (A318624)

Name: Anti-TIM 3 Antibody [DM83] - BSA and Azide free
Brand: Antibodies.com
SKU: A318624
Price: 665.0 USD
Availability: InStock

Bild Vergrößern

$665

Monoklonal [DM83] Kaninchen Antikörper gegen TIM 3 für ELISA und Flow Cytometry.

100% Qualitätsgarantie

Preis-Match-Garantie

Versand

Versandkosten

$40

Vorlaufzeit

Lieferung in 7-8 Werktagen

Kontakt

Telefon

+1 (314) 370-6046

orders@antibodies.com

Garantien

100% Qualitätsgarantie

Preis-Match-Garantie

Stichworte:

Rekombinant Antikörper

Produktdetails

Produktname

Anti-TIM 3 Antibody [DM83] - BSA and Azide free

Beschreibung

Recombinant rabbit monoclonal [DM83] antibody to TIM 3.

Anwendungen

ELISA, Flow Cytometry

Verdünnungen

ELISA: 1:5,000-10,000, Flow Cytometry: 1:100

Reaktivität

Human

Wirt

Rabbit

Klonalität

Monoclonal

Klon

DM83

Isotyp

IgG

Konjugat

Unconjugated

Reinigung

Affinity chromatography.

Konzentration

Reconstitution dependent.

Produktform

Lyophilized

Rekonstitution

Reconstitute with distilled sterile water.

Formulierung

Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.

Lagerung

Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.

Allgemeine Hinweise

Prior to reconstitution, centrifuge the vial at 5,000g for 3-5 minutes at room temperature. Reconstitute with appropriate volume of distilled sterile water to bring product to 1mg/ml concentration. After addition of distilled sterile water, mix by gentle tapping. Note: It is not recommended to vortex or vigorously pipette the sample.

Synonyme

CD366, FLJ14428, HAVcr-2, Havcr2, HAVR2_HUMAN, Hepatitis A virus cellular receptor 2, Kidney injury molecule 3, KIM 3, KIM3, T cell immunoglobulin and mucin domain containing 3, T cell immunoglobulin mucin 3, T-cell immunoglobulin and mucin domain-containing protein 3, T-cell immunoglobulin mucin family member 3, T-cell immunoglobulin mucin receptor 3, T-cell membrane protein 3, TIM-3, TIM3, TIMD-3, TIMD3

Isotyp-Kontrollantikörper

Kompatible Sekundärantikörper

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

Produktbilder (3)

Bild Vergrößern

Figure 1: Anti-TIM 3 Antibody [DM83] - BSA and Azide free (A318624)

ELISA plates were pre-coated with Recombinant Human TIM 3 Protein (Fc Chimera 6xHis Tag) (A318378) at 2 µg/ml (100 µl/well) which can bind Anti-TIM 3 Antibody [DM83] - BSA and Azide free (A318624) in a linear range of 0.32-40 ng/ml.

Bild Vergrößern

Figure 2: Flow Cytometry - Anti-TIM 3 Antibody [DM83] - BSA and Azide free (A318624)

Expi 293 cell line was transfected with irrelevant protein (A) and human TIM3 (B) were surface stained with Anti-TIM 3 Antibody [DM83] - BSA and Azide free (A318624) at 1µg/ml followed by Anti-Rabbit IgG Antibody (Alexa 488).

Bild Vergrößern

Figure 3: Flow Cytometry - Anti-TIM 3 Antibody [DM83] - BSA and Azide free (A318624)

Flow cytometry analysis of antigen binding of Anti-TIM 3 Antibody [DM83] - BSA and Azide free (A318624). (A) Anti-TIM 3 Antibody [DM83] - BSA and Azide free (A318624) does not bind to 293T cells that do not express TIM3. (B) A clear peak shift of Anti-TIM 3 Antibody [DM83] - BSA and Azide free (A318624) was seen compared to the control when incubated with TIM3-expressing 8226 cells, indicating strong binding of Anti-TIM 3 Antibody [DM83] - BSA and Azide free (A318624) to TIM3. Antibodies were incubated at 10 µg/ml.