Anti-Thrombin Aptamer [HD1-15dA] (Biotin) (A321232)

Anti-Thrombin Aptamer [HD1-15dA] (Biotin)

Synthetic DNA aptamer to Thrombin [HD1-15dA] (Biotin).

Biotin

Resuspend to 100µM with 10mM Tris-HCl, pH 7.5, and 0.1mM EDTA.

Assay Buffer: Phosphate Buffered Saline, pH 7.4, with 3 mM Magnesium Chloride and 1mg/ml Bovine Serum Albumin. Binding Temperature: 25°C.

5'-dGpdGpdTpdTpdGpdGpdTpdGpdTpdGpdGpdTpdTpdGpdGpdApdApdApdApdApdApdApdApdApdApdApdApdApdApdAp-3'

30 nt

9,424.2 Da

Affinity (Kd) = 50.9 nM

HPLC

Lyophilized

Reconstitution dependent.

Lyophilized from nuclease-free water (no residual salts).

Shipped at 4°C. Lyophilized: Store at -20°C. Reconstituted: Store short term at 4°C (i.e. use within days) or aliquot and store at -20°C (medium term: i.e. use within weeks) or -80°C long term. Avoid freeze/thaw cycles.

Anti-Thrombin Aptamer [HD1-15dA] (Biotin) (A321232) is a replica of the aptamer described in PMID: 17990265.

Aptamers have not been validated by Antibodies.com and they need to be tested/evaluated under identical conditions to which they were reported in the literature including, but not limited to, temperature and matrix/buffer conditions. All aptamers are manufactured on request and have a lead time of 30-45 business days.