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Anti-Thrombin Aptamer [D2-5] (Biotin) (A320923)

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Secondary Structure - Anti-Thrombin Aptamer [D2-5] (Biotin) (A320923) - Antibodies.com
$730
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Name
Anti-Thrombin Aptamer [D2-5] (Biotin)
Description
Synthetic DNA aptamer to Thrombin [D2-5] (Biotin).
Conjugate

Biotin

Aptamer Resuspension
Resuspend to 100µM with 10mM Tris-HCl, pH 7.5, and 0.1mM EDTA.
Apatmer Binding Conditions
Buffer for Clotting Inhibition Assay: Tris-HCl Buffer, pH 8, with 5mM Potassium Chloride and 100mM Sodium Chloride. Binding Temperature: 37°C.
Sequence
5'-dApdGpdCpdGpdApdGpdTpdApdGpdGpdTpdTpdGpdGpdTpdGpdTpdGpdGpdTpdTpdGpdGpdGpdGpdCpdTpdCpdGpdCpdTp-3'
Aptamer Length
31 nt
Aptamer Molecular Weight (Da)
9,710.34 Da
Extinction Coefficient
295700 M-1cm-1
Purification
HPLC
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from nuclease-free water (no residual salts).
Storage
Shipped at 4°C. Lyophilized: Store at -20°C. Reconstituted: Store short term at 4°C (i.e. use within days) or aliquot and store at -20°C (medium term: i.e. use within weeks) or -80°C long term. Avoid freeze/thaw cycles.
Product Notes
Anti-Thrombin Aptamer [D2-5] (Biotin) (A320923) is a replica of the aptamer described in PMID: 17016675.
General Notes
Aptamers have not been validated by Antibodies.com and they need to be tested/evaluated under identical conditions to which they were reported in the literature including, but not limited to, temperature and matrix/buffer conditions. All aptamers are manufactured on request and have a lead time of 30-45 business days.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Secondary Structure - Anti-Thrombin Aptamer [D2-5] (Biotin) (A320923) - Antibodies.comBild Vergrößern
Figure 1: Anti-Thrombin Aptamer [D2-5] (Biotin) (A320923)
Secondary structure of Anti-Thrombin Aptamer [D2-5] (Biotin) (A320923), with a dG of -4.41 kcal/mol.
PubMed LogoPublikation anzeigen
Analysis of the evolution of the thrombin-inhibiting DNA aptamers using a genetic algorithm
Verweise: Anti-Thrombin Aptamer [D2-5] (Biotin) (A320923)
Abstrakt:

We previously identified a thrombin-inhibiting DNA aptamer that was presumed to form a G-quartet structure with a duplex. To investigate the importance of the sequences in the duplex region and to obtain aptamers with higher inhibitory activities, we randomized the sequences of the duplex region of this aptamer and carried out selection based on inhibitory activity using a genetic algorithm. This method consisted of selection via an inhibition assay, crossover, and mutation in silico. After two cycles, we obtained ligands with greater inhibitory activities than that of the original aptamer. In addition, the duplex sequences were found to contribute to the inhibitory activities of aptamers.

Publikation anzeigen
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