This antibody reacts with the C-terminus of TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. The staining with Ab-1 is completely blocked by the peptide used for raising this antibody. TGF (aa50) is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.
Anwendungen
ELISA, IF, IHC-P
Verdünnungen
IF: 1-2 µg/ml, IHC-P: 2-4 µg/ml
Reaktivität
Human, Bovine, Rat, Mouse
Immunogen
Synthetic peptide corresponding to 17 amino acids from the C terminal of rat TGF alpha. The exact sequence is proprietary.
Wirt
Mouse
Klonalität
Monoclonal
Klon
MF9
Isotyp
IgG1
Leichte Ketten
kappa
Konjugat
Unconjugated
Reinigung
Protein A/G chromatography.
Konzentration
200 µg/ml
Molekulargewicht
~6 kDa
Produktform
Liquid
Formulierung
Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.
Lagerung
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
SDS-PAGE analysis of Anti-TGF alpha Antibody [MF9] under non-reduced and reduced conditions; showing intact IgG and intact heavy and light chains, respectively. SDS-PAGE analysis confirms the integrity and purity of the antibody.
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