Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Lagerung
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyme
ALS10, OTTHUMP00000002171, OTTHUMP00000002172, OTTHUMP00000002173, TADBP_HUMAN, TAR DNA binding protein, TAR DNA binding protein 43, TAR DNA-binding protein 43, TARDBP, TDP 43, TDP-43
Figure 1: Western Blot - Anti-TDP43 Antibody [ARC0492] (A306079)
Western blot analysis of extracts of various cell lines, using Anti-TDP43 Antibody [ARC0492] (A306079) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-TDP43 Antibody [ARC0492] (A306079) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded rat ovary using Anti-TDP43 Antibody [ARC0492] (A306079) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human esophageal using Anti-TDP43 Antibody [ARC0492] (A306079) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence analysis of HeLa cells using Anti-TDP43 Antibody [ARC0492] (A306079) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of NIH-3T3 cells using Anti-TDP43 Antibody [ARC0492] (A306079) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of C6 cells using Anti-TDP43 Antibody [ARC0492] (A306079) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Chromatin immunoprecipitation (ChIP) analysis of extracts of K562 cells, using Anti-TDP43 Antibody [ARC0492] (A306079) and Rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.