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TBX1 Kit Elisa (A104280)

$670
Versandkosten
Vorlaufzeit
Lieferung in 5-8 Werktagen
Telefon
+1 (314) 370-6046
Mo bis Frei, 8am - 4pm AST
Email
orders@antibodies.com
Produktname
TBX1 ELISA Kit
Assay-Typ
Indirect
Assay-Prinzip
This TBX1 ELISA Kit applies the qualitative technique of an indirect ELISA. Streptavidin is bound to the immunoassay plate and specific biotinylated double stranded (dsDNA) oligonucleotides are then added to bind to the streptavidin via a high affinity biotin-streptavidin interaction. After subsequent blocking of extraneous binding sites in each well, the sample containing the target of interest can be added. Primary antibody is added to bind activated transcription factors bound to the dsDNA oligonucleotide, which has been immobilized via the plate-coated streptavidin. A HRP-conjugated secondary antibody specific for rabbit IgGs is added, which allows for specific binding to the Primary Antibody, and consequently colorimetric detection upon addition of the TMB substrate. For color development, TMB (3, 3’, 5, 5’-Tetramethylbenzidine) is added to each well. After addition of the substrate, a peroxidase catalyzed reaction will produce a blue TMB Diimine product that is proportional to the target concentration in the sample. Color development is quenched by addition of Stop Solution, or 2 N Sulfuric Acid, which turns the solution yellow. The absorbance can then be read by a spectrophotometer at 450 nm and subsequently allowing for determination of the target concentration in the sample.
Plattform
Microplate
Nachweismethode
Colorimetric
Reaktivität
Human
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Lagerung
Store at + 4°C. Stable for 6 months.
Synonyms
Brachyury, CAFS, CTHM, DGCR, DGS, DORV, T box, T box 1, T box 1 transcription factor, T box 1 transcription factor C, T box protein 1, T box transcription factor TBX 1, T box transcription factor TBX1, T-box protein 1, T-box transcription factor TBX1, TBX 1, TBX 1C, TBX1C, TBX1_HUMAN, Testis specific T box protein, Testis-specific T-box protein, TGA, VCFS
Haftungsausschluss
Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

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