Tabituximab Biosimilar - Anti-FZD10 Antibody - BSA and Azide free
Beschreibung
Recombinant chimeric monoclonal antibody to FZD10.
Anwendungen
ELISA, Flow Cytometry
Verdünnungen
ELISA: 1:5,000-10,000, Flow Cytometry: 1:100
Reaktivität
Human
Wirt
Chimeric
Klonalität
Monoclonal
Isotyp
IgG1
Konjugat
Unconjugated
Reinigung
Affinity chromatography.
Konzentration
Reconstitution dependent.
Produktform
Lyophilized
Rekonstitution
Reconstitute with distilled sterile water.
Formulierung
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization.
Lagerung
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Allgemeine Hinweise
Prior to reconstitution, centrifuge the vial at 5,000g for 3-5 minutes at room temperature. Reconstitute with appropriate volume of distilled sterile water to bring product to 1mg/ml concentration. After addition of distilled sterile water, mix by gentle tapping. Note: It is not recommended to vortex or vigorously pipette the sample.
ELISA plates were pre-coated with Recombinant Human FZD10 Protein (Fc Tag) (A318093) at 2 µg/ml (100 µl/well) which can bind Tabituximab Biosimilar - Anti-FZD10 Antibody - BSA and Azide free (A324693) in a linear range of 3.20-80 ng/ml.In order to specifically detect Tabituximab Biosimilar - Anti-FZD10 Antibody - BSA and Azide free (A324693), Mouse Anti-Human Fab Antibody was used as detection antibody.
Flow cytometry analysis of 1µg/ml of Tabituximab Biosimilar - Anti-FZD10 Antibody - BSA and Azide free (A324693) on Expi293 cells transfected with Human FZD10 protein (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
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