Synthetic Virus-like Particle Human Claudin18.2 Protein (EGFP Tag)
Beschreibung
Full-length transmembrane human Claudin18.2 protein (EGFP tag) expressed on the cell surface of Virus like particles.
Anwendungen
ELISA, FACS
Ausdruckssystem
HEK293 cells
Natur
Synthetic
Spezies
Human
Proteinlänge
Full length protein.
Molekulargewicht
Full length human Claudin18.2 protein has a MW of 54.2 kDa.
Konjugat
Unconjugated
Produktform
Lyophilized
Konzentration
Reconstitution dependent.
Formulierung
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Lagerung
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Haftungsausschluss
Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.
Figure 1: Synthetic Virus-like Particle Human Claudin18.2 Protein (EGFP Tag) (A318480)
ELISA plates were pre-coated with 0.5 µg/per well Synthetic Virus-like Particle Human Claudin18.2 Protein (EGFP Tag) (A318480). Serial diluted Anti-Claudin18.2 Chimeric Antibody [Zolbetuximab Biosimilar] - Azide free (A318887) solutions were added, washed, and incubated with secondary antibody before ELISA reading. From above data, the EC50 for Anti-Claudin18.2 Chimeric Antibody [Zolbetuximab Biosimilar] - Azide free (A318887) binding with CLDN18.2-EGFP-VLP is 877.5 µg/ml.
Figure 2: Synthetic Virus-like Particle Human Claudin18.2 Protein (EGFP Tag) (A318480)
FACS analysis of Synthetic Virus-like Particle Human Claudin18.2 Protein (EGFP Tag) (A318480): A. Negative Control 1: Expi293 cells were stained with Synthetic Virus-like Particle Human Claudin18.2 Protein (EGFP Tag) (A318480). B. Negative Control 2: Anti-GPRC5D-CAR-Expi293 cells (an irrelevant CAR) were stained with Synthetic Virus-like Particle Human Claudin18.2 Protein (EGFP Tag) (A318480). C. Positive Control: Anti-CLDN18.2-CAR-Expi293 cells were stained with biotin labeled Protein L, followed by Streptavidin-APC Antibody. D. Anti-CLDN18.2-CAR-Expi293 cells were stained with Synthetic Virus-like Particle Human Claudin18.2 Protein (EGFP Tag) (A318480).
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