Synthetic nanodiscs offer a stable and biologically relevant environment that closely mimics cell membranes and enables full-length transmembrane human T1R3 protein to be purified and analysed in vitro.
Anwendungen
SDS-PAGE, ELISA
Ausdruckssystem
HEK293 cells
Natur
Synthetic
Spezies
Human
Proteinlänge
Full length protein.
Molekulargewicht
Full length human T1R3 protein has a MW of 93.4 kDa.
Konjugat
Unconjugated
Produktform
Lyophilized
Konzentration
Reconstitution dependent.
Formulierung
Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% - 8% trehalose is added as protectants before lyophilization.
Lagerung
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Synonyme
G protein coupled receptor, GPCR TAS1R3, Sac, Saccharin preference, Sweet taste receptor T1R3, T1 R3, T1R 3, TAS1 R3, TAS1R 3, TAS1R3, Taste receptor type 1 member 3, Taste receptor type 1 member 3 precursor, TR 3, TR3, TS1R3_HUMAN
Haftungsausschluss
Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.
Figure 1: Synthetic Nanodisc Human T1R3 Protein (A325301)
ELISA plates were pre-coated with Synthetic Nanodisc Human T1R3 Protein (A325301) at 0.2µg/per well. Serial diluted Anti-Flag Monoclonal Antibody solutions were added, washed, and incubated with a secondary antibody before reading the ELISA. From the above data, the EC50 for Anti-Flag Monoclonal Antibody binding with Synthetic Nanodisc Human T1R3 Protein (A325301) is 4.332ng/ml.
Figure 3: Synthetic Nanodisc Human T1R3 Protein (A325301)
Diagram showing how synthetic nanodiscs containing full-length multi-pass transmembrane proteins in a phospholipid bilayer are generated from native cell membranes.
