Synthetic nanodiscs offer a stable and biologically relevant environment that closely mimics cell membranes and enables full-length transmembrane human GPCR GPR55 protein to be purified and analysed in vitro.
Ausdruckssystem
HEK293 cells
Natur
Synthetic
Spezies
Human
Proteinlänge
Full length protein.
Molekulargewicht
Full length human GPCR GPR55 protein has a MW of 36.6 kDa.
Konjugat
Unconjugated
Produktform
Lyophilized
Konzentration
Reconstitution dependent.
Formulierung
Lyophilized from nanodisc solubilization buffer (20mM Tris-HCl, 150mM NaCl, pH 8.0). Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Lagerung
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Synonyme
G protein coupled receptor 55, G-protein coupled receptor 55, GPR 55, Gpr55, GPR55_HUMAN, LPIR1, Probable G protein coupled receptor 55
Haftungsausschluss
Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.
Figure 1: Synthetic Nanodisc Human GPCR GPR55 Protein (A317348)
ELISA plates were pre-coated with Synthetic Nanodisc Human GPCR GPR55 Protein (A317348) at 0.2µg/per well. Serial diluted Anti-Flag Monoclonal Antibody solutions were added, washed, and incubated with a secondary antibody before reading the ELISA. From the above data, the EC50 for Anti-Flag Monoclonal Antibody binding with Synthetic Nanodisc Human GPCR GPR55 Protein (A317348) is 11.86ng/ml.
