Synthetic nanodiscs offer a stable and biologically relevant environment that closely mimics cell membranes and enables full-length transmembrane human GPCR GPR40 protein to be purified and analysed in vitro.
Anwendungen
ELISA, SDS-PAGE
Ausdruckssystem
HEK293 cells
Natur
Synthetic
Spezies
Human
Proteinlänge
Full length protein.
Molekulargewicht
Full length human GPCR GPR40 protein has a MW of 31.5 kDa.
Konjugat
Unconjugated
Produktform
Lyophilized
Konzentration
Reconstitution dependent.
Formulierung
Lyophilized from nanodisc solubilization buffer (20mM Tris-HCl, 150mM NaCl, pH 8.0). Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Lagerung
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Figure 1: Synthetic Nanodisc Human GPCR GPR40 Protein (A318419)
ELISA plates were pre-coated with Synthetic Nanodisc Human GPCR GPR40 Protein (A318419) (0.2 µg/well). Serial diluted Anti-Flag Tag Antibody solutions were added, washed, and incubated with secondary antibody before ELISA reading. From this data, the EC50 for Anti-Flag Tag Antibody binding with FFAR1-Nanodisc is 4.566 µg/ml.
Figure 3: Synthetic Nanodisc Human GPCR GPR40 Protein (A318419)
Diagram showing how synthetic nanodiscs containing full-length multi-pass transmembrane proteins in a phospholipid bilayer are generated from native cell membranes.
